ifn β cat 8499 if 010 cf Search Results


human ifn β  (R&D Systems)
96
R&D Systems human ifn β
Human Ifn β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B2+cat+8499+if+010+cf/Recombinant+Human+IFN-beta+Protein/pmc10817939-325-2-15
Average 96 stars, based on 1 article reviews
human ifn β - by Bioz Stars, 2026-09
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ifnβ  (R&D Systems)
96
R&D Systems ifnβ
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Ifnβ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B2+cat+8499+if+010+cf/Recombinant+Human+IFN-beta+Protein/pmc11221667-223-23-26
Average 96 stars, based on 1 article reviews
ifnβ - by Bioz Stars, 2026-09
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recombinant human ifn-gamma protein  (Bio-Techne corporation)
96
Bio-Techne corporation recombinant human ifn-gamma protein
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Recombinant Human Ifn Gamma Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
recombinant human ifn-gamma protein - by Bioz Stars, 2026-09
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human ifn-beta duoset elisa  (Bio-Techne corporation)
95
Bio-Techne corporation human ifn-beta duoset elisa
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Human Ifn Beta Duoset Elisa, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B2+cat+8499+if+010+cf/Human+IFN-beta+DuoSet+ELISA/bio-techne+corporation___dy814-05
Average 95 stars, based on 1 article reviews
human ifn-beta duoset elisa - by Bioz Stars, 2026-09
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recombinant mouse ifn-gamma protein  (Bio-Techne corporation)
97
Bio-Techne corporation recombinant mouse ifn-gamma protein
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Recombinant Mouse Ifn Gamma Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
recombinant mouse ifn-gamma protein - by Bioz Stars, 2026-09
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recombinant human il-29/ifn-lambda 1 protein  (Bio-Techne corporation)
95
Bio-Techne corporation recombinant human il-29/ifn-lambda 1 protein
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Recombinant Human Il 29/Ifn Lambda 1 Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B2+cat+8499+if+010+cf/Recombinant+Human+IL-29%2FIFN-lambda+1+Protein/custom%401598-il%40us11672829
Average 95 stars, based on 1 article reviews
recombinant human il-29/ifn-lambda 1 protein - by Bioz Stars, 2026-09
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recombinant human ifn-beta protein, cf  (Bio-Techne corporation)
94
Bio-Techne corporation recombinant human ifn-beta protein, cf
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Recombinant Human Ifn Beta Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B2+cat+8499+if+010+cf/Recombinant+Human+IFN-beta+Protein%2C+CF/bio-techne+corporation___8499-if-cf
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recombinant human ifn-beta protein, cf - by Bioz Stars, 2026-09
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recombinant human ifn-gamma protein, cf  (Bio-Techne corporation)
93
Bio-Techne corporation recombinant human ifn-gamma protein, cf
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Recombinant Human Ifn Gamma Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B2+cat+8499+if+010+cf/Recombinant+Human+IFN-gamma+Protein%2C+CF/bio-techne+corporation___285-if-cf
Average 93 stars, based on 1 article reviews
recombinant human ifn-gamma protein, cf - by Bioz Stars, 2026-09
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np0007 human ifn beta elisa kit pbl assay science  (PBL Assay)
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PBL Assay np0007 human ifn beta elisa kit pbl assay science
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Np0007 Human Ifn Beta Elisa Kit Pbl Assay Science, supplied by PBL Assay, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human ifn-alpha a (alpha 2a) protein  (Bio-Techne corporation)
95
Bio-Techne corporation recombinant human ifn-alpha a (alpha 2a) protein
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Recombinant Human Ifn Alpha A (Alpha 2a) Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human ifn-alpha a (alpha 2a) protein - by Bioz Stars, 2026-09
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a8233  (ApexBio)
90
ApexBio a8233
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
A8233, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a8233 - by Bioz Stars, 2026-09
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clarity western ecl substrate bio rad  (Bio-Rad)
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Bio-Rad clarity western ecl substrate bio rad
( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or <t>IFNβ</t> stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 <t>cells</t> <t>stimulated</t> with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.
Clarity Western Ecl Substrate Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or IFNβ stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 cells stimulated with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.

Journal: PLOS Biology

Article Title: SAMD9L acts as an antiviral factor against HIV-1 and primate lentiviruses by restricting viral and cellular translation

doi: 10.1371/journal.pbio.3002696

Figure Lengend Snippet: ( A ) Western blot analyses of endogenous SAMD9L expression under basal, IFN⍺, or IFNβ stimulation in various cells: 293T cells, immortalized T cell line SupT1, immortalized fibroblastic cell lines (TZM-bl, HeLaP4P5), PBLs from 3 donors, and primary macrophages from 2 donors. The last line of the first blot shows the condition of KD HelaP4P5 cells stimulated with IFNβ and transduced with either shControl (shCD241) or shSAMD9L (performed at day 0 of the viral replication experiment). ( B ) Experimental setup for the KD and viral replication experiments. ( C ) HIV-1 T/F pCH077 replication assay in shControl or shSAMD9L HeLaP4P5 +IFNβ over 6 days of replication. Titration was performed using the TZM-bl assay. Y-axis represents the raw RLU results for 1 experiment with the technical replication duplicates for each condition. Three independent biological replicates were performed for HIV-1 pCH077 and pCH077:VSVg, and 2 for LAI . ( D ) The mean of 3 independent experiments is shown for HIV-1 pCH077 replication in shSAMD9L conditions normalized to shControl. **, p -value < 0.01. The data and the raw images underlying this Figure can be found in and Raw Images. HIV-1, human immunodeficiency virus type 1; IFN, interferon; ISG, interferon-stimulated gene; KD, knock-down; PBL, primary blood lymphocyte; RLU, relative luminescence unit; SAMD9L, sterile alpha motif domain-containing protein 9-like; T/F, transmitted/founder.

Article Snippet: To generate HelaP4P5 SAMD9L KD and control (CD241) KD, the cells were plated at 0.2M cells/ml in a 6-well plate and stimulated with IFNβ (500 IU/ml, R&D Systems cat. 8499-IF-010).

Techniques: Western Blot, Expressing, Transduction, Titration, Virus, Knockdown, Sterility